lewis lung carcinoma cell line 3ll  (ATCC)

Journal: Cancers

Article Title: Regulation of Constitutive Interferon-Stimulated Genes (Isgs) in Tumor Cells Contributes to Enhanced Antitumor Response of Newcastle Disease Virus-Infected Tumor Vaccines

doi: 10.3390/cancers10060186

Figure Lengend Snippet: Newcastle disease virus (NDV)-infected tumor vaccines (NDV-TV) model and induction of antitumor response by rNDV-TV. ( a ) The schematic of NDV-TV model was shown. The tumor cells were infected with rNDV in vitro, irradiated by ultraviolet for inactivation of rNDV and tumor cells. Then rNDV-TV was administered to mice as immunogen. rNDV-TV ( n = 3), UV-TV (ultraviolet irradiated tumor vaccine) ( n = 3), or each medium ( n = 2) were administered to mice and splenic mononuclear cells (SMCs) were co-cultured with UV-irradiated tumor cells for 5 days. After cytotoxic T cell (CTL) induction, SMCs were harvested and co-cultured with target tumor cells for 24 h. The cytotoxicity was measured using quantifying lactate dehydrogenase (LDH) in the supernatant (* p < 0.05, ** p < 0.01). rNDV-TV and UV-TV were prepared using ( b ) B16 and ( c ) SCC VII. Medium was administered to the control mice.

Article Snippet: The murine fibrosarcoma cell line WEHI164 (laboratory stock cell line), mammary tumor cell line BALB-MC (derived from BALB/c mice, #JCRB0233.0, JCRB cell bank, Ibaraki, Japan), melanoma cell line B16 and Lewis lung carcinoma cell line 3LL (derived from C57BL/6, #JCRB0202 and # JCRB1348, JCRB cell bank, Ibaraki, Japan), squamous cell carcinoma SCC VII (provided from Dr. Inanami, Hokkaido University, Sapporo, Japan), and bladder carcinoma MBT-2 (derived from C3H/HeN, # IFO50041, JCRB cell bank, Ibaraki, Japan) were used.

Techniques: Virus, Infection, Vaccines, In Vitro, Irradiation, Cell Culture, Control

Journal: Cancers

Article Title: Regulation of Constitutive Interferon-Stimulated Genes (Isgs) in Tumor Cells Contributes to Enhanced Antitumor Response of Newcastle Disease Virus-Infected Tumor Vaccines

doi: 10.3390/cancers10060186

Figure Lengend Snippet: rNDV infection rate in tumor cells after ruxolitinib treatment. ( a ) Tumor cells were infected with rNDV (MOI of 2) after ruxolitinib (0~1.0 μg/mL) treatment for 20 h. The infection rate was calculated from the GFP expressing cell ratio observed via fluorescent microscopy (* p < 0.05, ** p < 0.01). ( b ) GFP expressed in (i) B16, (ii) WEHI164, (iii) 3LL, (iv) SCCVII, (v) MBT-2, and (vi) BALB-MC infected with rNDV after ruxolitinib (left: 0 μg/mL, right: 1.0 μg/mL) treatment (×100 magnification).

Article Snippet: The murine fibrosarcoma cell line WEHI164 (laboratory stock cell line), mammary tumor cell line BALB-MC (derived from BALB/c mice, #JCRB0233.0, JCRB cell bank, Ibaraki, Japan), melanoma cell line B16 and Lewis lung carcinoma cell line 3LL (derived from C57BL/6, #JCRB0202 and # JCRB1348, JCRB cell bank, Ibaraki, Japan), squamous cell carcinoma SCC VII (provided from Dr. Inanami, Hokkaido University, Sapporo, Japan), and bladder carcinoma MBT-2 (derived from C3H/HeN, # IFO50041, JCRB cell bank, Ibaraki, Japan) were used.

Techniques: Infection, Expressing, Microscopy